VIDEO - Membrane Proteins Workshop - The baculovirus/insect cells system with Jan H Driller

VIDEO - Membrane Proteins Workshop - The baculovirus/insect cells system with Jan H Driller

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Speaker: Jan H Driller, Aarhus University, Denmark

This lecture was part of the workshop, Structural Resolution of Membrane Proteins: From Expression to Sample, organised in May 2021 by the LINXS working group Membrane Proteins, which fall under the Integrative Structural Biology theme.

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Abstract

Mammalian membrane proteins are notoriously difficult to express. In contrast to bacteria or yeast, the baculovirus/insect cells system provides a good alternative to express these proteins in higher eukaryotes. Insect cells provide post-translational modifications, such as disulfide bonds or glycosylation, as well as a more akin membrane lipid composition compared to bacterial or yeast expression systems. Insect cells grow to high cell densities and using the baculovirus system even multi-protein complexes can be co-expressed.